We investigated glucose concentrations from 8-20 mM to assess the concentration of adrenaline that completely abolishes c events. We used confocal microscopy with a high spatial and temporal resolution to evaluate glucose-stimulated c events and their sensitivity to adrenaline. Here we report the effect of physiological adrenaline levels on c dynamics in beta cell collectives in mice pancreatic tissue slice preparation. In most studies unphysiologically high adrenaline concentrations have been used to evaluate the role of adrenergic stimulation in pancreatic endocrine cells. The stimulation of α2-adrenergic receptors on the plasma membrane of beta cells reduces their excitability and insulin secretion mostly through diminished cAMP production and downstream desensitization of late step(s) of exocytotic machinery to cytosolic Ca 2+ concentration ( c).
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